Lei Zhou’s Blog

Obtain an Image:

1. Start Up — Liquid Nitrogen; High Tension; Filament (3.8)
2. Beam Alignment (without a specimen in the holder or without inserting the holder) — 17,500x
   1. Gun Tilt (Maximise brightness of incident beam) — Focus beam (INTENSITY); Spread beam to ~3 cm diameter; Maximise screen brightness using MULTIFUNCTION X and Y; Recenter the beam using SHIFT X and Y
   2. Gun Shift (performed if changing the spot size produce a significant beam shift) — LOW DOSE and EXPOSURE MODE; Spot size 9; Focus beam (INTENSITY) and center spot using SHIFT X and Y; Spot size 3 (or 1&2); Center spot using MULTIFUNCTION X and Y
   3. Condenser Aperture Alignment — The center of the beam do not move as we change magnification
   4. Loading a Specimen
   5. Insert a Specimen
   6. Searching for an Area of Interest
   7. Setting Eucentric Height
   8. Check the Beam Tilt Alignment at 100,000x and if necessary do a further alignment
   9. Condenser Beam Astigmatism — Make the beam as circular as possible OR Obtain a cleanest, roundest de-saturated filament image
3. Image Alignment — 100,000x
   1. Pivot Point Alignment (performed if a shift of the beam is accompanied by a tilt, or vice versa; OBJECTIVE APERTURE can be used to protect your specimen) — Set the magnification to ~ Bring the two beams together
   2. Rotation Center Alignment (including VOLTAGE CENTERING or CURRENT CENTERING; performed if the image moves out of center during focusing) — FOCUS STEP 5 or 6 –> FOCUS STEP 7 or 8; minimise image movement using MULTIFUNCTION X and Y
   3. Image shift (performed if changing the magnification setting produce movement of the beam or of the image) — High SA magnification –> Highest SA magnification –> High M magnification –> lowest M magnification; Focus image and center beam; center the image feature with MULTIFUNCTION X and Y
   4. Coma x,y Pivot Point Alighment — Coma free, then PP coma x,y superimpose beams
   5. Coma Free — match image contrast for both tilt
4. Centering the OBJECTIVE LENS APERTURE
5. Focusing an Image — Focusing an image using the WOBBLER pushbutton (optional) — Adjust the direction of the wobbler to be perpendicular to the direction of the structures to be focussed using the MULTIFUNCTION X; Insert a specimen, adjust the height and focus the image; Press D pushbutton; Select a camera length of approximately 600 nm; Focus the illumination using the INTENSITY knob; Ensure that the objective aperture is correctly centered; Press WOBBLER pushbutton to get two spots within the area of the objective aperture using MULTIFUNCTION Y; Press D pushbutton and focus the image to minimal blur; Press WOBBLER pushbutton to switch off
6. Fine Adjusting an Image — Image (Objective) Astigmatism — Amorphous area; 100,000x; Live FFT; Concentric circles
7. Setting Exposure Time
8. Take a Picture
9. End Up: High Tension; Filament

Obtain a Diffraction Pattern

1. Diffraction Alignment — Camera length from shortest to longest, Focus diffraction pattern (FOCUS) and center central beam spot using MULTIFUNCTION X and Y
2. Diffraction Shift — Center diffraction pattern using MULTIFUNCTION X and Y
3. Correcting diffraction pattern astigmatism in SA diffraction mode — Obtain a TEM BF image of a specimen in SA magnification; Remove specimen and objective aperture from the beam; Press D and adjust the MAGNIFICATION knob to obtain the required camera length; Set SPOT SIZE to 4; Turn INTENSITY knob until low illumination intensity is obtained on the screen; Adjust FUCOS until the diffraction crossover image is obtained; Press STIG button and try to get a symmetrical 3-pointed image using MULTIFUNCTION X and Y

Obtain a Microdiffraction Pattern

1. Center selected area of specimen on screen
2. Choose appropriate spot size (C1); Focus beam to crossover (C2) and positon on selected area
3. Go to D; remove OA
4. Vary C2 aperture to change size of diffraction disks. For every small beamsize/selected areas it may be necessary to slightly reposition beam or recenter aperture after changing C2 aperture. Observe in M mode to determine if this is necessary
5. Do not insert SAD aperture

X-ray Analysis

1. Locate the region of interest in the center of the TEM viewing screen
2. Remove the objective aperture
3. Insert Condenser aperture 3. Align the Aperture
4. Tilt the specimen toward the detector by increasing the ą-tilt to approximately +20^o
5. Reduce the spot size to spot size 4
6. Position the beam over the area of interest using INTENSITY and BEAM SHIFT Knobs
7. Insert the EDS detector by pressing the button on the software window
8. Make the COUNT RATE >1000cps <4000>

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Recently, I read a lot of articles talking about online storages and online softwares. It looks like hard drives are not needful anymore and a WebOS era is coming. Indeed, I believe the WebOS will take the place of windows and become the mainstream finally. But when is the final date, I don’t know. The realisation of this dream depends on the next generation of internet. For current internet, it is difficult to accomplish this task. Nobody would like to wait a long time to get a software startup. At the moment, all online softwares look like childish, no really useful online softwares have been available. Not a software can really take the place of windows office. Google docs just a kind of WordPad. It can not used to accomplish really work, such as a thesis.

I think FaceBook have start a good kickoff by open its API to public. People can develop various small tools for it. The model is good, but the ability is not enough. As what I have mentioned above, not a really useful tools can be used by experts.

In the future, I think people needn’t buy software for computers, they may buy a lot of service online, such as a service providing Windows Office abililty. We don’t need worry about security and update, all thingks will be done by the compapy who provide the service. One doesn’t need hard drive, they buy online storage services. The company backup all files for him every minute. He don’t need worry about the loss of data. We also buy movie and musics online and save it online. People can go everywhere, when they need use software, they just need to computer to login their services.

Who can provide the platform, who will be next google in the world. A few articles, I thought useful:

Google, Microsoft and Apple building online storage havens: you win

ONLINE BUSINESS TOOLBOX: 230+

No More Hard Drive! 100 Free Software Apps to Go Online-Only